Figure 4.

β-Galactoside activity from lacZ reporter gene fusions. Results from fusions to initiation regions (A) with both possible translation initiation codons (wild type), (B) with upstream initiation codon mutated, (C) with downstream initiation codon mutated, and (D) with both initiation codons mutated. The construct with the new annotated initiation codon mutated is marked with an asterisk. Upper left panel shows the structures of the gene fusions with an X marking mutated sites. Lower right panel shows TetRP (black), transcription (gray), and DNA sequence near the yigE initiation codon. -35 and -10 regions of the possible promoter are highlighted (red). The 2006 ATG initiation codon and transcription start site are enlarged. This figure is available in black and white in print and in colour at DNA Research online.