Figure 7.

Overexpression of CaWRKY50 in tobacco leads to early flowering and senescence. (A) The top and side view of early flowering and senescence phenotype in representative CaWRKY50 overexpressing T₀ lines (B2, B3, X1 and Y1) with respect to pBI121 vector transformed controls (BV). The dwarf phenotype with chlorotic lesions on leaves seen in T₀ plants were not retained in T₂ generation (B). The senescence phenotype was quantified at 19th week after germination by measuring the total chlorophyll content of T₂ generation line (B2, B3, P1, and Y1), BV, and wild-type tobacco leaves. The total chlorophyll content correlates with the level of CaWRKY50 expression in transgenic lines. The data presented here were derived from the means of three biological replicates with each replicate having a minimum of 10 plants for each line. Error bars represent ±SD of means. *Indicates significant difference (P < 0.001) when compared with wild type or blank vector control. This figure is available in black and white in print and in colour at DNA Research online.