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Autoradiogram of an 8.0% nondenaturing polyacrylamide gel showing gel shifts for purified MgrA and SarA proteins with a 268-bp sarV promoter fragment. Mobility of the DNA band in the presence of increasing amounts of either MgrA or SarA protein is indicated on the top. Cold competition lanes with a 10-fold excess (molar ratio) of the unlabeled 268-bp sarV fragment are indicated and contained 1.0 μg of the purified MgrA or SarA protein.
