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. 2016 Jun 15;11(6):e0157543. doi: 10.1371/journal.pone.0157543

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© 2016 Shi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — All mice were dark adapted for at least 6 hours before ERG recordings. (A) The average scotopic ERG a-wave amplitudes recorded from miR-150^-/- with HFD (miR-150^-/--HFD) are significantly lower compared to the WT fed with normal chow diet (WT-Normal; *) or miR-150^-/- fed with normal chow diet (miR-150^-/--Normal; #). There is no statistical difference between WT fed with HFD (WT-HFD) and miR-150^-/--HFD. HFD-mice (both WT and miR-150^-/- groups) had significantly smaller (v) a-wave amplitudes compared to mice fed with a normal chow (both WT and miR-150^-/- groups). The miR-150^-/- mice (both normal chow and HFD groups) had significantly smaller (w) a-wave amplitudes compared to the WT mice (both normal chow and HFD groups). (B) The averaged photopic ERG a-wave amplitudes recorded from WT-HFD are significantly lower than WT-Normal (*) at 3 and 10 cd.s/m² light intensities. The photopic a-wave amplitudes recorded from miR-150^-/--HFD are significantly lower than WT-normal (#) at 3, 10, and 25 cd.s/m² light intensities. HFD-mice (both WT and miR-150^-/- groups) had significantly smaller (v) amplitudes compared to mice fed with a normal chow (both WT and miR-150^-/- groups). (C) The average scotopic ERG b-wave amplitudes recorded from miR-150^-/--HFD are significantly lower compared to WT-Normal (*) or miR-150^-/--Normal (#). There is no statistical difference between WT-HFD and miR-150^-/--HFD. HFD-mice (both WT and miR-150^-/- groups) had significantly smaller (v) a-wave amplitudes compared to mice fed with a normal chow (both WT and miR-150^-/- groups). The miR-150-/- mice (both normal chow and HFD groups) had significantly smaller (w) a-wave amplitudes compared to the WT mice (both normal chow and HFD groups). (D) The averaged ERG photopic b-wave amplitudes recorded from WT-HFD are significantly lower than WT-Normal (*) and miR-150^-/--Normal (#) at 1 and 10 cd.s/m² light intensities. The photopic b-wave amplitudes recorded from miR-150^-/--HFD are significantly different from the other 3 groups (&) at 25 cd.s/m² light intensities. HFD-mice (both WT and miR-150^-/- groups) had significantly smaller (v) amplitudes compared to mice fed with a normal chow (both WT and miR-150^-/- groups). (E) The averaged scotopic oscillatory potential amplitudes [as a summation from OP1 to OP4; Ʃ(OP1-4)] recorded from HFD-mice (both WT-HFD and miR-150^-/--HFD) are significantly lower (*) than mice fed with a normal chow (both WT-Normal and miR-150^-/--Normal) at 0.1, 0.3, 10, and 25 cd.s/m² light intensities. (F) The averaged photopic oscillatory potential amplitudes [Ʃ(OP1-4)] recorded from HFD-mice (both WT-HFD and miR-150^-/--HFD) are significantly lower (*) than mice fed with a normal chow (both WT-Normal and miR-150^-/--Normal) at 25 cd.s/m² light intensities. (A-F) Overall, there is no statistical significance of interaction between two factors: miR-150 null mutation and HFD regimen (2-way ANOVA). p < 0.05 (denoted as *, #, &, v, w). Data of scotopic and photopic ERG a- and b-waves and OPs are listed in Tables 1 and 2.