TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli | ||
| DH10B | F−mcrA D(mrr-hsdRMS-mcrBC) F80dlacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara, leu)7697 galU galK λ−rpsL endA1 nupG | Gibco/BRL Corp. |
| HB101 | supE44 ara14 galK2 lacY1 Δ(gpt-proA)62 rpsL20 (Strr)xyl-5 mtl-1 recA13 Δ(mcrC-mrr) HsdS-(r− m−) | D. Ohman |
| C. perfringens | ||
| NCTC 8798 | CPE+ | C. Duncan |
| SM101 | High-frequency electroporation derivative of NCTC 8798 | 51 |
| SM120 | ccpA mutant derivative of SM101 | This study |
| Plasmids | ||
| pBluescript SK− | Stratagene Corp. | |
| pBluescript SK+ | Stratagene Corp. | |
| pSF3 | Source of mob region | D. Ohman |
| pJIR750 | C. perfringens-E. coli shuttle vector | 7 |
| pJIR751 | C. perfringens-E. coli shuttle vector | 7 |
| pYZ67 | pBluescript SK-/catP | This study |
| pSM300 | pBluescript SK-/ermBP | This study |
| pSM300M | pSM300/mobilization (mob) region from pSF3 | This study |
| pSM310 | pBluescript SK-/ccpA | This study |
| pSM310Cm | pSM310/catP | This study |
| pSM225 | pSM300M/ccpA-catP | This study |
| pSM237 | pJIR751/cpe-gusA fusion | This study |
| pSM257 | pJIR751/ccpA | This study |
| pDOB13 | pJIR751/ccpA | This study |