FIG. 6.

Cif is composed of a C-terminal effector domain and an exchangeable N-terminal translocation signal. (A) Production and secretion of chimeric Cif-TEM fusions. Molecular mass markers (in kilodaltons) are indicated to the left. (B) Translocation in HeLa cells of chimeric Cif-TEM fusions. The presented data are averages of triplicate values of the results from two independent experiments. (C) CPE triggered by chimeric Cif-TEM fusions. HeLa cells were infected under conditions used to monitor translocation. At the end of the interaction, bacteria were killed with gentamicin and HeLa cells were incubated for a further 3 days. Actin and nuclei were stained, respectively, with rhodamine-phalloidin and DAPI. Bars, 10 μM.