TABLE 1.
List of strains and plasmids
| Strain or plasmid | Description and/or genotype | Reference or expressed protein |
|---|---|---|
| Strains | ||
| E22 | Rabbit EPEC, serotype O103:H2 | 34 |
| E22 Δcif | E22 Δcif::Kan | 26 |
| E22 espB | E22 espB::Kan | 34 |
| E22 escN | E22 escN::Kan | 26 |
| E22 ΔcesT | E22 ΔcesT::Kan | This study |
| Plasmids | ||
| pCX340 | pBR322 derivative, cloning vector used to fuse EPEC effectors to the mature form of TEM-1 β-lactamase | TEM |
| pCX311 | Negative control, fusion of MBP to TEM-1 | MBP-TEM |
| pCX312 | Negative control, fusion of GST to TEM-1 | GST-TEM |
| pCX302 | Fusion of Tir to TEM-1 | Tir-TEM |
| pCX329 | Residues 1 to 26 of Tir fused to TEM-1 | Tir1-26-TEM |
| pCX303 | Fusion of Map to TEM-1 | Map-TEM |
| pCX304 | Fusion of EspF to TEM-1 | EspF-TEM |
| pCX313 | Fusion of Cif to TEM-1 | Cif-TEM |
| pCX327 | Residues 1 to 16 of Cif fused to TEM-1 | Cif1-16-TEM |
| pCX315 | Residues 1 to 32 of Cif fused to TEM-1 | Cif1-32-TEM |
| pCX316 | Residues 1 to 62 of Cif fused to TEM-1 | Cif1-62-TEM |
| pCX317 | Residues 1 to 86 of Cif fused to TEM-1 | Cif1-86-TEM |
| pCX318 | Residues 1 to 124 of Cif fused to TEM-1 | Cif1-124-TEM |
| pCX319 | Residues 1 to 150 of Cif fused to TEM-1 | Cif1-150-TEM |
| pCX320 | Residues 1 to 206 of Cif fused to TEM-1 | Cif1-206-TEM |
| pCX321 | Residues 1 to 256 of Cif fused to TEM-1 | Cif1-256-TEM |
| pCX322 | Naturally truncated Cif of PMK5 fused to TEM-1 | CifPMK5-TEM |
| pCX323 | Naturally truncated Cif of E2348/69 fused to TEM-1 | CifE2348-TEM |
| pCX351 | Cif-TEM fusion with AMG tripeptide at position 21 | CifAMG-TEM |
| pCX352 | Tir-TEM fusion with AMG tripeptide at position 21 | TirAMG-TEM |
| pCX353 | Map-TEM fusion with AMG tripeptide at position 21 | MapAMG-TEM |
| pCX354 | EspF-TEM fusion with AMG tripeptide at position 21 | EspFAMG-TEM |
| pCX361 | Residues 1 to 20 of Cif fused to TEM-1 | Cif1-20-TEM |
| pCX362 | Residues 1 to 20 of Tir fused to TEM-1 | Tir1-20-TEM |
| pCX363 | Residues 1 to 20 of Map fused to TEM-1 | Map1-20-TEM |
| pCX364 | Residues 1 to 20 of EspF fused to TEM-1 | EspF1-20-TEM |
| pCX371 | Residues 21 to 282 of Cif fused to TEM-1 | CifΔ(1-20)-TEM |
| pCX351 | Residues 1 to 20 of Cir fused to CifΔ(1-20)-TEM | Cif1-20-CifΔ(1-20)-TEM |
| pCX372 | Residues 1 to 20 of Tir fused to CifΔ(1-20)-TEM | Tir1-20-CifΔ(1-20)-TEM |
| pCX373 | Residues 1 to 20 of Map fused to CifΔ(1-20)-TEM | Map1-20-CifΔ(1-20)-TEM |
| pCX374 | Residues 1 to 20 of EspF fused to CifΔ(1-20)-TEM | EspF1-20-CifΔ(1-20)-TEM |