Fig. 2.

(A) Fluorescent microscopic image of cells in peritoneal washing from a patient with pancreatic cancer, demonstrating large, round, strongly eGFP positive cells, and rare, weakly eGFP positive cells with dendritic processes (10 ×). The cells were subsequently sorted by eGFP expression. The weakly eGFP positive cells were counter-stained with a fluorescent antibody to a human leukocyte antigen (HLA-DR), confirming them to be dendritic cells (B; 100 ×). Conversely, the strongly eGFP positive cells counter-stained with an antibody to CEA (C; 100 ×). eGFP positive cells isolated from a patient with metastatic gastrointestinal stromal tumor (GIST) were fixed and stained with an antibody to c-KIT (D; 100 ×).