Figure 5. Dye coupling between KCs and β’2mp neurons.
(A) Z stack of MB lobes and half of the peduncle. Biocytin-filled neurites (arrowhead) are observed within the boundary of the α’/β’ lobe (dotted line) and peduncle (PED). (B–D) The lobe layers were divided into 3 parts from anterior to posterior and then stacked. In each stack, biocytin-filled neurites are visible within the boundaries of theα’/β’ lobe and peduncle. (E) Z stack of KC somas, the calyx and half of the peduncle. One biocytin-filled fiber is seen Green, biocytin-loaded neurites; magenta, the fluorescence of YFP-labeled KCs (driven by MB247-lexA); blue, the fluorescence of td-Tomato-labeled MBONs (driven by MB011B-GAL4). Arrow, arborizations of β’2mp neurons projecting to the tip of the β’ lobe; arrowhead, the biocytin-filled KC fibers; dotted line, the boundary of the α’/β’ lobe. PED, peduncle. Scale bar, 30 μm. (F) Schematic diagram of the brain. White, the α’/β’ neuron that was dye-coupled with the β’2mp neurons. “*”, the presumed points of gap junctions.