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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: J Cell Biochem. 2016 Jan 21;117(8):1813–1821. doi: 10.1002/jcb.25481

Figure 2. Bag5 expression regulates ER stress signaling.

Figure 2

A. Expression analysis of Bag5 in cardiomyocytes infected with Ad-Bag5 at increasing MOI of adenovirus. B. Immunocytochemistry suggests that Bag5 is predominantly localized to the perinuclear space. NRVCs were transfected with Ad-Null or Ad-Bag5 for 48h and cells were fixed with 4% PFA. Fixed cells were probed with Bag5 (green), Flag (red), and Actinin (red). C. Western blot analysis shows that adenovirus mediated expression of Bag5 upregulates sXBP1. D. Western blot analysis shows that adenovirus mediated expression of Bag5 upregulates the unfolded protein response (UPR) in NRVCs. NRVC cells were infected with Ad-Bag5 for the 72h and then Western blots were done with PDI, ATF4, ATF6, Grp78, ELFα and pELFα antibodies.