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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Cell Rep. 2016 Jun 2;15(11):2449–2461. doi: 10.1016/j.celrep.2016.05.032

FIGURE 1. TLR4 activates CASPASE-8 to eliminate CYLD.

FIGURE 1

(A–B) BMDMs from wild type mice were treated with 100 ng/ml of LPS for the indicated times (A) or with the indicated concentrations of LPS for 4 hours (B). (C) BMDMs from wild type BMDMs were infected with viable E. coli or C. rodentium for 4 hours. (D) BMDMs from wild type, Ripk3−/−, and Ripk3−/−Casp8−/− mice were stimulated with either LPS (100 ng/ml), E. coli (MOI 1), or C. rodentium (MOI 1) for the indicated times. Lysates were subject to western blot analysis using CYLD (D1A10), RIPK3, CASPASE-8, and GAPDH antibodies. All experiments shown are representative of at least three independent experiments with similar results.