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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Cell Rep. 2016 Jun 2;15(11):2449–2461. doi: 10.1016/j.celrep.2016.05.032

FIGURE 2. TLR4-TRIF signaling and not TNF mediates CASPASE-8 dependent cleavage of CYLD.

FIGURE 2

(A) Wild type and Tnf−/− BMDMs were treated with 100 ng/ml of LPS for 4 hours. (B–C) Wild type, Myd88−/−, and Trif−/− BMDMs were treated with 10 ng/ml of LPS for the indicated times. (D) Wild type BMDMs were pre-treated with 30 μM Necrostatin-1 prior to stimulation with LPS for 4 hours. (E) Immortalized BMDMs generated from RosaCreERT2Ripk1fl/fl mice were treated with ethanol or with tamoxifen (4-OHT) to delete RIPK1. Cells were then treated with 100 ng/ml of LPS for the indicated times. Lysates were blotted as indicated. All experiments shown are representative of at least three independent experiments with similar results.