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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Cell Rep. 2016 Jun 2;15(11):2449–2461. doi: 10.1016/j.celrep.2016.05.032

FIGURE 5. TLR4-induced auto-necroptosis in macrophages also requires type I IFN.

FIGURE 5

Ifnar1+/+ and Ifnar1−/− BMDMs were pre-treated with 25 μM zVAD-fmk prior to stimulation with LPS (A), viable E. coli or C. rodentium (B), or murine TNF (C) for 24 hours. (D) Wild type BMDMs were treated with isotype control or 1 ug/ml of IFNAR1 neutralizing antibody prior to stimulation with the indicated concentrations of LPS for 24 hours. Cell death in (A–D) was measured by propidium iodide staining or by CellTiter-Glo as in Figure 3. * p<0.05, ** p<0.01, *** p<0.001. (E) Wild type BMDMs were treated as in (D) prior to stimulation with LPS for 24 hours. Lysates were blotted with antibodies to phosphorylated STAT1 and total STAT1. Experiment shown is representative of at least three experiments performed independently.