Skip to main content
. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Am Chem Soc. 2016 Jun 2;138(23):7353–7364. doi: 10.1021/jacs.6b02960

Figure 4.

Figure 4

Characterization of the selective modification of overexpressed GFP-CRABP2 by probe 4 in HEK293T cells. (a) Alkyne-functionalized arylfluorosulfate probe 4 bearing a biphenyl moiety. The fluorosulfate group is shown in red. (b) GFP fluorescence of the overexpressed and probe-labeled GFP-CRABP2 and FABP5-GFP analyzed by native PAGE. The modification of GFP-CRABP2 by probe 4 caused a significant band-shift of GFP-CRABP2 to higher molecular weight. The corresponding Coomassie-stained gel is shown in Figure S21. (c) In-gel fluorescence and western blot analysis of the modified GFP-CRABP2 and FABP5-GFP after cell lysis and incorporation of rhodamine-N3 using CuAAC Click. The corresponding Coomassie-stained gels are shown in Figure S22.