Figure 6.
Inhibition of CRABP2/RARα target gene induction by probe 4. (a) mRNA transcript levels of the RA signaling target gene CRBP1 as measured by qPCR in MCF-7 cells. MCF-7 cells in charcoal-treated FBS were pretreated with 20 µM 4 or DMSO for 4 h and subsequently treated with 100µM RA or EtOH for 24 h. (b-c) mRNA transcript levels of CRABP2 (b) and FABP5 (c) in MCF-7 cells treated under the same conditions as in (a). Error bars show SEM (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001.