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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Apr 17;1860(7):1519–1527. doi: 10.1016/j.bbagen.2016.04.009

Fig. 1.

Fig. 1

Effect of PPNO (100 μM) on Topoisomerase IIα. The decatenation (A) assay was carried out using kDNA as described in the Methods section. Lane 1, control DNA with 100μM PPNO; lanes 2 and 3 with topo IIα (2 and 5 units, respectively); lanes 4 and 5, with topo IIα (2 and 5 U, respectively) and 100μM PPNO; lanes 6 and 7, with topo IIα (2 and 5 units, respectively) preincubated with 100μM PPNO (10 min) before adding DNA. DNA relaxation (B) assay using pHOT1 DNA was carried out as described in the Methods section. Lane 1, control DNA; lane 2, with 100μM PPNO; lanes 3 and 4, with topo IIα (5 and 10 units, respectively); lanes 5 and 6, with topo IIα (5 and 10 units, respectively) and 100μM PPNO; lanes 7 and 8, with topo IIα (5 and 10 units, respectively) preincubated with 100μM PPNO (10 min) before adding DNA. NC, nicked open circular kDNA; CC, closed circular kDNA; ORI, origin.