Table 1.
Primer type | Primer sequence (5′ to 3′ orientation) | Expected amplicon size (bp) | Usage | |
---|---|---|---|---|
Exon 1 | Outer forward primer | GCGAGATTCAAACCCAGGAT | 822 | PCR |
Outer reverse primer | GCCGTGATATTACTATCAATCCC | PCR | ||
Nested forward primer | GGGTAAAGGAAGAGGGTTGG | 545 | PCR | |
Nested reverse primer | TAAGAACAGACATATTAGACGAGT | PCR and sequencing | ||
Sequencing forward primer | TATATATGAAATATTATAACATTAC | - | sequencing | |
Exon 2 | Forward primer | ACTTCAACCAATCCACAAATCC | 672 | PCR and sequencing |
Reverse primer | AGTCCATTCATTCAATGTTTCAA | PCR and sequencing |