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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Int J Parasitol. 2016 Apr 9;46(7):417–424. doi: 10.1016/j.ijpara.2016.03.006

Table 1.

Primer sequences used for PCRs and sequencing of the two exons of the Schistosoma mansoni SmSULT-OR gene in this study.

Primer type Primer sequence (5′ to 3′ orientation) Expected amplicon size (bp) Usage
Exon 1 Outer forward primer GCGAGATTCAAACCCAGGAT 822 PCR
Outer reverse primer GCCGTGATATTACTATCAATCCC PCR
Nested forward primer GGGTAAAGGAAGAGGGTTGG 545 PCR
Nested reverse primer TAAGAACAGACATATTAGACGAGT PCR and sequencing
Sequencing forward primer TATATATGAAATATTATAACATTAC - sequencing
Exon 2 Forward primer ACTTCAACCAATCCACAAATCC 672 PCR and sequencing
Reverse primer AGTCCATTCATTCAATGTTTCAA PCR and sequencing