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Enzyme activity of wild and mutant type recombinant PgluE8 E55A, PgluE8 D116A, PgluE8 C23G, PgluE8 C364G and PgluE8 C23G-C364G
| Enzyme | Activity (U/mg) | Relative activity (%)a |
|---|---|---|
| Wild type PgluE8 | 5.86 ± 0.23 | 100.00 |
| PgluE8 E55A | 0.52 ± 0.12 | 8.87 |
| PgluE8 D116A | 0.71 ± 0.09 | 12.12 |
| PgluE8 C23G | 8.60 ± 0.30 | 148.08 |
| PgluE8 C364 | 8.24 ± 0.13 | 140.46 |
| PgluE8 C23G-C364G | 9.10 ± 0.42 | 155.28 |
aValues represent the mean ± SD (n = 3) relative to the untreated control samples
