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. 2016 Jul;126:63–70. doi: 10.1016/j.biochi.2016.02.010

Fig. 1.

Fig. 1

Diminished expression of Mthfd1L in curly tail embryos. (A) Mthfd1L mRNA abundance is significantly lower in the caudal region of E10.5 (28–29 somite stage) ct/ct embryos (n = 6) and ct/ctTgGrhl3 (n = 3) than in stage-matched +ct/+ct embryos (n = 3; p < 0.001, ANOVA). The cranial region of E9.5 ct/ct embryos also exhibits lower abundance of Mthfd1L mRNA, which is not corrected in ct/ctTgGrhl3 embryos (n = 2 per group, p < 0.05). In contrast, Grhl3 expression is diminished in the E9.5 cranial region of ct/ct embryos compared with +ct/+ct (p < 0.001; ANOVA), and is normalised by the presence of a Grhl3-containing BAC (ct/ctTgGrhl3). (B) Immunoblots reveal a 104 kDa band corresponding to Mthfd1L that is less abundant in ct/ct samples (caudal region at E10.5) than stage-matched +ct/+ct samples. Immunoblot for Gapdh (37 kDa) is used as loading control. (C) Analysis of a second group of E10.5 ct/ct (n = 5) and +ct/+ct (n = 3) samples replicated the finding of reduced Mthfd1L expression in ct/ct (p < 0.01). In contrast, no difference in Mthfd1L expression was detected between wild-type (n = 6) and Grhl3 null (n = 7) embryos.