Figure 1.

T cells stimulated by allogeneic DC do not exhibit Th1/Th2 bias in cytokine production. Human pan T cells from multiple donors were cultured in vitro with allogeneic DC that had been activated with LPS and IFN-γ. (A) Time-course analysis by MSD of cytokine levels accumulated in MLR supernatants; panels clockwise from the left: IFN-γ, TNF-α, IL-5, and IL-13; filled shapes represent T cells cultured with DC, open shapes represent T cells cultured alone (three donors). Cells collected from day 6 of MLR were analyzed for (B) IFN-γ spot forming units by ELISPOT or (C) CD4⁺ and CD8⁺ production of IFN-γ and IL-5 by flow cytometry. Contour plots representative of three independent experiments after fluorescence compensation and gating on live cells → singlets → CD3⁺ T cells → CD4⁺ versus CD8⁺ T cells.