Table 1.

Immune checkpoint expression on dividing and non-dividing CD4⁺/CD8⁺ T cells.

	CD4	CD8
Undivided (mean ± SEM)
CD25	28.45 ± 1.83	15.10 ± 1.84
	30.96 ± 1.25	12.76 ± 0.61
	26.37 ± 0.26	9.09 ± 0.37
	20.67 ± 0.12	12.20 ± 0.81
PD-1	5.48 ± 0.10	7.22 ± 0.08
	10.54 ± 0.19	12.40 ± 0.49
	7.92 ± 0.25	13.23 ± 0.84
	8.95 ± 0.41	16.90 ± 0.71
LAG-3	2.31 ± 0.15	1.21 ± 0.15
	1.06 ± 0.07	2.96 ± 0.74
	3.84 ± 0.08	1.78 ± 0.29
	3.42 ± 0.19	4.39 ± 0.61
TIM-3	1.01 ± 0.13	1.78 ± 0.23
	1.04 ± 0.14	1.54 ± 0.08
	0.72 ± 0.10	1.30 ± 0.28
	0.07 ± 0.06	1.14 ± 0.16
Divided (mean ± SEM)
CD25	97.19 ± 0.15	97.44 ± 0.20
	97.68 ± 0.03	96.45 ± 0.15
	99.17 ± 0.48	97.47 ± 0.59
	94.43 ± 1.05	85.00 ± 2.02
PD-1	47.72 ± 1.52	21.22 ± 1.38
	59.46 ± 0.73	24.19 ± 1.28
	60.50 ± 2.14	26.13 ± 1.60
	78.03 ± 2.08	52.47 ± 0.45
LAG-3	30.89 ± 2.07	19.91 ± 0.42
	37.72 ± 1.73	27.12 ± 2.28
	37.53 ± 1.27	71.00 ± 1.16
	49.13 ± 1.03	80.20 ± 0.79
TIM-3	57.84 ± 2.44	80.12 ± 0.94
	61.63 ± 2.93	86.74 ± 0.59
	39.40 ± 1.88	58.03 ± 2.28
	56.57 ± 1.66	78.87 ± 1.30

Human pan T cells were stimulated with allogeneic DC for 6 days. CD4⁺ and CD8⁺ T cells were analyzed by flow cytometry and gated on responding (divided) or non-responding (undivided) population based on proliferation dye dilution, and analyzed for CD25, PD-1, LAG-3, and TIM-3 expression. Mean and SEM were calculated from three biological replicates per donor. Data from four donors are shown.