Fig. 4.

Protease protection assay of GFP fused to a C-terminal proteolysis tag and an N-terminal targeting peptide in the presence and absence of BMCs. Total lysates were analysed by SDS-PAGE and subsequently western blotted with an anti-GFP primary antibody. Cell densities were normalised to an OD₆₀₀=2.5 for loading of samples. The faint bands seen in lanes 2-6 may, in part, be a result of unspecific binding, as a faint band is also seen in lane 1 (shell proteins only, no GFP).