Table 2.

Effect of the HCM related TnT K280N mutation on Ca²⁺-sensitivity and phosphorylation dependence of Ca²⁺-sensitivity using recombinant TnT K280N (see Fig. 2). X = exchanged. All data rounded to 2 significant figures.

	Experiment	EC50, μM		Ratio (EC50 Test/Control)	N	p	TnI Phosphorylation
	Test vs Control	Test	Control				Test	Control
A	Donor vs DonorXK280N	0.17 ± 0.037	0.10 ± 0.020	×1.7 ± 0.12	6	0.0028	1.6 ± 0.11	1.6 ± 0.11
B	DonorXK280N vs dpDonorXK280N	0.10 ± 0.0050	0.098 ± 0.0050	×1.1 ± 0.037	10	0.11	1.6 ± 0.060	0.34 ± 0.19
C	Donor v 50% DonorXK280N	0.14 ± 0.018	0.089 ± 0.0080	×1.6 ± 0.13	4	0.018	1.6 ± 0.11	1.6 ± 0.11