Skip to main content
. 2016 Jun 16;7:947. doi: 10.3389/fmicb.2016.00947

FIGURE 4.

FIGURE 4

DNA, membrane and fat body visualization of gently (PBS-Tween80) and shock (PBS) starved M. smegmatis. (A,B) Log-phase cultures were subjected to gentle and shock starvation. Samples were collected over time and stained with DAPI (blue) to visualize DNA and FM4-64 (red) to visualize membranes. (A) Fluorescence microscopy imaging of samples taken during the first 24 h of starvation. (B) Confocal microscopy imaging of DAPI and FM4-64 stained log-phase and 14-day-old starved bacilli, and the corresponding DIC images. Arrows indicate apparent septa. (C) Confocal microscopy imaging of Nile red (green) stained log-phase bacilli and 14-day-old starved bacilli to visualize fat bodies. Corresponding DIC images are shown in the lower panel. Fields shown are representative.