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. 2016 Jun 16;7:743. doi: 10.3389/fpls.2016.00743

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Copyright © 2016 Alkanaimsh, Karuppanan, Guerrero, Tu, Hashimoto, Hwang, Phu, Arzola, Lebrilla, Dandekar, Falk, Nandi, Rodriguez and McDonald.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Map of gene constructs. (A) pTRBO-prBChE-KDEL, (B) pTRBO-prBChE, and (C) p35S-P19, 35S: Cauliflower Mosaic Virus (CaMV) promotor, RAmy3DSP: rice alpha-amylase 3D gene signal peptide, Replicase: replicase gene of tobacco mosaic virus (TMV), MP: movement protein, BChE: codon optimized sequences for the human butyrylcholinesterase gene. 3XFLAG: specific amino acid codon sequences used for immunodetection and purification of the protein. KDEL: Codons encoding lysine, aspartic acid, glutamic acid, leucine, specifying the endoplamsmic retention sequence. P19: P19 gene from Tomato Bushy Stunt Virus (TBSV).