FIGURE 2.

Quantification of active prBChE protein in crude extracts using TRBO viral system as a function of sublocalization in plant cell and extraction methodology. A modified Ellman assay was used to determine the accumulation level of active prBChE protein species localized in different sub-compartments in plant cells in N. benthamiana leaves agroinfiltrated with TRBO expression system. The apoplast-targeted prBChE was extracted either by leaf homogenization or collecting the AWF. Specific activity was determined as the ratio of active protein estimated from the Ellman assay relative to total soluble protein estimated by Bradford assay. All data plotted are the average of three independent measurements ± SD.