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(A) SDS-PAGE and (B) Western blot of different variants of prBChE protein. (A) Coomassie stained gel with 7 μg total soluble protein (TSP) of prBChE-AWF (lanes 1) and total leaf extract of prBChE (lanes 2) loaded into each well under non-reduced conditions. Lane M shows the pre-stained protein molecular weight standards along with the molecular weight in kDa. (B) Western blot analysis using 1:2,500 anti-FLAG HRP conjugated antibody.
