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. 2016 Jun 16;7:862. doi: 10.3389/fpls.2016.00862

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Copyright © 2016 Ampah-Korsah, Anderberg, Engfors, Kirscht, Nordén, Kjellström, Kjellbom and Johanson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasma membrane localization of NbXIP1;1α in P. pastoris. Western blot analysis of membrane fractions of P. pastoris cells expressing the N-terminally His-tagged NbXIP1;1α protein. In accordance with previous results using the anti-(His)₄ antibody (Nordén et al., 2011), the blot shows a pattern that is characteristic of the expressed protein. IM, internal membranes fraction. PM, plasma membrane fraction. The fractions were diluted to the same volume and an equal volume was loaded in the two lanes.