Fig. 4.

Immunolocalization of H3Ser10ph (a–e), CENH3 (f–j), and α-tubulin in meiosis of 1Rv(1A)xR, 5R(5D)xR, and 6R(6A)xR hybrids. Equational plus reductional type of chromosome behaviour. a–c, f, g Metaphase I, different meiocytes. f _i The same cell as f, DAPI counterstained. g _i The same cell as g, DAPI counterstained. d Anaphase II. e Telophase II. h Anaphase I. h _i The same cell as h, DAPI counterstained. i Anaphase II. i _i The same cell as i, DAPI counterstained. j Telophase II. j _i The same cell as j, DAPI counterstained. DNA blue, H3Ser10ph and CENH3 labelling red, α-tubulin labelling green. Bars represent 10 μm