Fig. 5.

Immunolocalization of H3Ser10ph (a–h), CENH3 (i–l), and α-tubulin in meiosis of 1Rv(1A)xR, 5R(5D)xR, and 6R(6A)xR hybrids. Equational type of chromosome behaviour. a–d Prometaphase I, different meiocytes. e Metaphase I. f Anaphase I. g Late anaphase I. h Telophase I. i, j Metaphase I. i _i, j _i The same cell as i, j, DAPI counterstained. k Anaphase I. k _i The same cell as k, DAPI counterstained. l Telophase I. l _i the same cell as l, DAPI counterstained. DNA blue, H3Ser10ph and CENH3 labelling red, α-tubulin labelling green. Bars represent 10 μm