FoxQ2 defines the central ANE territory, where it activates sFRP1/5 and Dkk3. (A) (a,b) six3 is expressed in a ring of ANE cells surrounding the central anterior pole of the 24 hpf embryo. (c,d) foxq2 expression around the anterior pole of the embryo. (e-h) Embryos injected with a FoxQ2 morpholino express six3 throughout the ANE territory (compare e,f with a,b). six3 and foxq2 (g,h) expression is expanded towards the vegetal/posterior pole in FoxQ2 morphants. AV, anterior view. (B) (a-d) Injecting a FoxQ2 morpholino into embryos completely eliminates sfrp1/5 and dkk3 expression. (e) qPCR analysis confirms downregulation of sfrp1/5 and dkk3 transcripts in FoxQ2 morphants. Microarray results are shown for comparison. MO, morpholino. Scale bars: 20 µm.