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Expression of sfrp1/5, dkk3 and foxq2 transcripts overlaps during ANE restriction. (Aa-d) foxq2 expression is first detected at the 32- to 60-cell stage in anterior/animal blastomeres (Aa shows a 60-cell embryo). (Ba-d) sfrp1/5 expression is first detected at the 120-cell stage within the anterior/animal half of the embryo. (Ca-d) dkk3 expression is first detected in 15-16 hpf blastula embryos within the anterior/animal half of the embryo. mes., mesenchyme. Scale bars: 20 µm.
