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. 2016 May 1;143(9):1523–1533. doi: 10.1242/dev.128165

Fig. 6.

Fig. 6.

ANE restriction depends on additive inputs from sFRP1/5 and Dkk3. (A) foxq2 mRNA expression marks the inner ANE territory in control (a,e), sFRP1/5 morphants (b,f), Dkk3 morphants (c,g), and sFRP1/5 plus Dkk3 double morphants (d,h). (a-d) Representative images showing the distribution of foxq2 mRNA expression in inner ANE of control and knockdown embryos. Scale bars: 20 µm. (B) The angle α shown in Aa-d was measured for ∼24 embryos from three batches using ImageJ. Volume=0.5(1–cos α/2) was used to calculate the percentage of the surface area (±s.e.m.) occupied by the inner ANE territory in control, sFRP1/5 morphants, Dkk3 morphants, and sFRP1/5 plus Dkk3 double morphants. MO, morpholino.