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. 2016 May 1;143(9):1475–1481. doi: 10.1242/dev.132209

Fig. 4.

Fig. 4.

HLCs can be replated following ASGR1 MACS enrichment and retain hepatocyte characteristics. (A) Immunocytochemical staining for hepatocyte markers in PHHs and replated ASGR1 MACS-enriched HLCs. CK18, cytokeratin 18 (keratin 18, type I). Staining was performed 72 h after replating. Scale bar: 100 μm. (B) Representative assays of hepatocyte cellular functions. Assays were performed at the following time points: unsorted HLCs, day 28 of differentiation; replated HLCs, 72 h after replating on day 25 of differentiation; PHHs, 48 h after plating. n=4 per cell line for HLCs and n=6 per cell line for replated HLCs. Cryopreserved PHHs were thawed and plated (n=4). Error bars represent s.e.m. **P<0.01, ***P<0.001, Student's t-test.