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Programmed cell death in the SCG is eventually complete by P20 in Plxna3−/− mice. (A) Tyrosine hydroxylase (TH) immunolabeling was performed on serial sections of SCG from P20 Plxna3+/+ (left) and Plxna3−/− (right) mice. (B) The total number of TH+ neurons in each SCG was ascertained by counting the number of TH+ cells in every third section throughout the entire ganglia (n=3 for both genotypes). There was no significant difference by t-test. Mean±s.e.m.
