Table 1.
Primer sets used for RT-qPCR.
| Gene name | F/R | Sequence (5′ to 3′) |
|---|---|---|
| Internal standard | ||
| 18S rRNA | F | TTCCGATAACGAACGAGACTCT |
| R | TGGCTGAACGCCACTTGTC | |
| Mitochondrial biogenesis | ||
| PGC1α | F | CCAAACCCACAGAAAACAGG |
| PPAR-gamma coactivator 1-alpha | R | TGGGGTCATTTGGTGACTCT |
| NRF1 | F | GAACTGCCAACCACAGTCAC |
| Nuclear respiratory factor 1 | R | TCGTCTGGATGGTCATTTCA |
| mtTFA | F | CCGAAGTGTTTTTCCAGCAT |
| Mitochondrial transcriptional factor A | R | GGCTGCAATTTTCCTAACCA |
| Antioxidant system | ||
| Nrf2 | F | GGGAGAAAACGACAGAAACC |
| Nuclear factor erythroid 2 related factor 2 | R | TGGGAGAGTAAGGCTTTCCA |
| HO-1 | F | TGACACCTGAGGTCAAGCAC |
| Heme oxygenase-1 | R | TCCTCTGTCAGCATCACCTG |
| NQO1 | F | AAACGTCTGGAAACCGTCTG |
| Nadph dehydrogenase quinone 1 | R | TTCTGCTCCTCTTGAACTTCC |
| Cat | F | GGACGCTCAGCTTTTCATTC |
| Catalase | R | TTGTCCAGAAGAGCCTGGAT |
| GPx | F | CTCATGACCGACCCCAAGTA |
| Glutathione peroxidase | R | CCCACCAGGAACTTCTCAAA |
| Mn-SOD | F | TCTGTGGGAGTCCAAGGTTC |
| Manganese superoxide dismutase | R | TAAGGCCTGTTGTTCCTTGC |
| Cu-SOD | F | GAGACCTGGGCAATGTGACT |
| Copper superoxide dismutase | R | TCATGGACCACCATTGTACG |