Figure 4.

In vitro characterization of pituitary adenoma cell cultures for mesenchymal stromal cell markers. Representative FACS histograms of pituitary somatotroph adenoma cell culture (a–f). Cells were positive for CD90 (a), CD105 (b), and CD44 (c) and were negative for CD34 (d), CD45 (e), and HLA-DR (f). Representative immunostaining of VIM (green) (g) and negative control (h). Nuclei were stained with DAPI (blue). 200x magnification. CD90, Thy-1 cell surface antigen; CD105, endoglin; CD44, CD44 molecule (Indian blood group); CD34, CD34 molecule; CD45, protein tyrosine phosphatase, receptor type, C; HLA-DR, major histocompatibility complex, class II, DR.