Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fed-batch culture analysis of cell growth (a), pH of broth (b) and nisin production (c) with F44, transformants with single expression of murG and combinatorial expression of hdeA, hdeB murG and ldh genes, respectively. The culture temperature was maintained at 30 °C. Concentrated sucrose (500 g/L) was fed at a constant rate of 2 mL/h between 6–12 h during fermentation process. Samples were taken at every 2 h.
