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. 2016 Jun 16;6:28192. doi: 10.1038/srep28192

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Copyright © 2016, Macmillan Publishers Limited

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Mice were intramuscularly injected with 1 × 10⁷ colony-forming units (CFUs) of C. perfringens Strain 13 (WT, n = 9), PLC-KO (KO, n = 9), or TGY medium as a control (uninfected, n = 9), bone marrow cells (BMCs) were isolated from mice after 24 hours, and flow cytometry analysis was performed using a Guava easyCyte. Representative flow cytometry profile (A), the absolute number of CD11b⁺Gr-1^high mature neutrophils per femur (B), the absolute number of CD11b⁺Gr-1^low immature neutrophils per femur (C), and bone marrow cellularity (D) are shown. Magnetically isolated Gr-1⁺ cells from BMCs were stained with Giemsa (E). One-way analysis of variance was employed to assess statistical significance.