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. 2016 Jun 16;6:28192. doi: 10.1038/srep28192

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A total of 1 × 10⁶ magnetically isolated Gr-1⁺ cells (n = 6 per condition) were cultured for 24 hours in the presence or absence (Control) of 100 ng/ml α-toxin (WT) or a variant α-toxin (H148G). Representative flow cytometry profile (A), the frequency and absolute number of CD11b⁺Gr-1^high neutrophils per culture well (B,C), and the frequency and absolute number of CD11b⁺Gr-1^low neutrophils per culture well (D,E) are shown. Values are mean ± standard deviation. One-way analysis of variance was employed to assess statistical significance.