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. 2016 Jun 16;6:28192. doi: 10.1038/srep28192

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A total of 5 × 10⁶ BMCs (n = 4 per condition) were cultured for 24 hours in the presence or absence (control) of 100 ng/ml α-toxin (α-toxin) and the indicated concentration of purified mouse G-CSF. Representative flow cytometry profile (A), the frequencies of CD11b⁺Gr-1^high neutrophils (B) and CD11b⁺Gr-1^low neutrophils (C) are shown. Magnetically isolated Gr-1⁺ cells from cultured BMCs were stained with Giemsa (D). Values are mean ± standard deviation. One-way analysis of variance was employed to assess statistical significance.