Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 16;6:28201. doi: 10.1038/srep28201

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

TGCs function was restricted in inactive p110δ. (A,B) Dual immunofluorescent test against p110δ and MMP12 on transverse sections of E8.5 tissue from p110δ^WT (n = 6) and p110δ^D910A/D910A (n = 6) female mice. (C) The statistical result of MMP12 expression in TGCs. (D,E) Immunohistochemistry test against MMP2 on transverse sections of E15.5 placenta from p110δ^WT (n = 6) and p110δ^D910A/D910A (n = 7) female mice. (F) The statistical result of MMP2 expression. (G,H) Bright-field images showed cultured EPC tissues from p110δ^WT (n = 3 mice) and p110δ^D910A/D910A (n = 4 mice) female mice, respectively. The TGCs that migrated out of original EPCs were indicated by red dotted lines in (G,H). (I) The statistical result of TGCs extension areas from the in vitro cultured EPC tissues. (J,K) The immunohistochemistry test against Ki67 on transverse sections of E15.5 placenta from p110δ^WT (n = 12) and p110δ^D910A/D910A (n = 11) female mice. The junctional zone, which was rich in SpT, was indicated with red dotted lines. (L) The statistical result of proliferation index. (M) RT-PCR results showed the mRNA expression of Hand1, Mash2, PL1, PL2, PLF, GATA3, LIFR, SOCS3 and MMP12 in deciduas at E8.5 and the placenta at E15.5 from p110δ^WT and p110δ^D910A/D910A female mice, respectively. (N,O) The quantitative analysis of genes mRNA expression. EPC, ectoplacental cone. The statistical data are expressed as the mean ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: P > 0.05. Scale bars: 25 μm (A,B), 50 μm (D,E,J,K), 200 μm (G,H).