Table 4. Primers used for LMP-1 sequence analysis and EBNA-2 typing.
| Primer sequence | Nested PCR run | |
|---|---|---|
| LMP-1 primers | ||
| LMP-168843R | CTACAACAAAACTGGTGGACT | 1st PCR |
| LMP-168039F | AGACAGTGTGGCTAAGGGAGT | |
| LMP-168810R | CTCCTTTGGCTCCTCCTGTT | 2nd PCR |
| LMP-168039F | AGACAGTGTGGCTAAGGGAGT | |
| LMP-168689R or LMP-168390R | CTGGCCATGAATCTGACTCT AGCGACTCTGCTGGAAATGAT | 3rd PCR |
| LMP-168075F | TGATTAGCTAAGGCATTCCCA | |
| EBNA-2 primers | ||
| EBNA-2-48810F | AGGGATGCCTGGACACAAGA | 1st PCR |
| EBNA-2-49411R | TGTGCTGGTGCTGCTGGTGG | |
| EBNA-2-1-Fa | TCTTGATAGGGATCCGCTAGGATA | 2nd PCR |
| EBNA-2-1-Ra | ACCGTGGTTCTGGACTATCTGGAC | |
| EBNA-2-2-Fb | CATGGTAGCCTTAGGACATA | 2nd PCR |
| EBNA-2-2-Rb | AGACTTAGTTGATGCCCTAG | |
Abbreviations: EBV, Epstein–Barr virus; EBNA-1, EBV nuclear antigen 1; LMP, latent membrane protein.
EBNA-2 type 1 EBV yields a 497 bp product.
EBNA-2 type 2 EBV yields a 162 bp product.