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. 2016 May 6;48(5):e231. doi: 10.1038/emm.2016.17

Figure 4.

Figure 4

Rac1 activation induces the nuclear translocation and activity of the STAT3 and NFκB complex. (a) HeLa cells expressing NT shRNA or Rac1 shRNA were incubated in HBSS for the indicated time periods and fluorescence-stained with anti-p65 Ab, anti-STAT3 Ab and DAPI. (b) The nuclear and cytoplasmic fractions were prepared from NT-HeLa cells and Rac1 shRNA-HeLa cells incubated in HBSS for the indicated time periods, and western blotted to detect pY705–STAT3, STAT3, p65, p50, histone H3 and tubulin. (c) HeLa cells stably expressing NT shRNA or Rac1 shRNA were incubated in HBSS for the indicated time periods, and STAT3 reporter activity and NFκB reporter activity were measured by real-time PCR. Data are presented as relative quantitation (RQ)±s.d. *P<0.05 and **P<0.01 compared with the respective control. Ab, antibody; DAPI, 4′,6-diamidino-2-phenylindole; HBSS, Hank's balanced salt solution; NT, non-target; shRNA, short hairpin RNA; NFκB, nuclear factor κB; STAT3, signal transducer and activator of transcription 3.