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. 2016 May 20;48(5):e234. doi: 10.1038/emm.2016.31

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Adipogenesis is inhibited by overexpression of FBXO9. Adipogenesis was induced using differentiation medium containing IBMX, dexamethasone and insulin either with or without rosiglitazone. Cells were transfected with Ad-GFP (100 MOI), Ad-FBXO9 (100 MOI) or Ad-FBXO9 ΔF (100 MOI) 2 days after induction of differentiation. (a) Cell lysates were harvested at the indicated day of adipogenesis and subjected to western blot analysis. (b) Cells were stained with oil red O at day 8 of adipogenesis. (c) Total RNA was harvested at the indicated day of adipogenesis, and PPARγ mRNA levels were determined by real-time PCR. The PPARγ mRNA level of the Ad-GFP-infected cells harvested on day 3 without rosiglitazone treatment was set as a reference value of 1, and others are presented as relative values (mean±s.e.m., n=4). G, Ad-GFP; F, Ad-FBXO9; Δ, Ad-FBXO9ΔF.