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. 2016 Jun 16;6:44. doi: 10.1186/s13578-016-0104-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The effect of NC on breast cancer parental cells and mammospheres viability was measured using MTT based. a, b NC had effects on cell proliferation of breast cancer MDA-MB-468 and MCF-7 parental cells and mammospheres in a concentration dependent manner. However, sphere-derived cells showed relative resistance to NC compared to parental cells. c, d Both Parental cells and mammospheres were treated with indicated concentrations of NC. However, no obviously cell death was observed by NC at 1.0 μM for 24, 48 and 72 h in MTT assay. *P < 0.05 compared with the control. The data are presented as the mean ± SD of three separate experiments