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. 2016 Jun 16;4:60. doi: 10.1186/s40478-016-0331-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — ATRX immunohistochemistry: a loss of immunoreactivity in necrotic/perinecrotic tumor areas, b Ambiguous ATRX status; areas with clear ATRX retention bordering areas with nuclear ATRX loss. c Diffuse astrocytoma grade II with ATRX loss, d Anaplastic Astrocytoma grade III with ATRX loss, e Oligodendroglioma grade II with ATRX retention, f Anaplastic Ependymoma with ATRX retention (f magnification ×200, a, c–e magnification ×100, b magnification ×40)