Table 6.

Fermentation data comparing strains ALK2, M1442, and M2886 in pH-controlled bioreactors in SSCF or SHF process configurations

Substrate	Mock hydrolysate	Mock hydrolysate	Mock hydrolysate	Pre-treated hardwood	Pre-treated hardwood	Pre-treated hardwood	Hardwood hydrolysate
Process type	SSCF	SSCF	SSCF	SSCF	SSCF	SSCF	SHF
Fermentation volume (liters)	1	1	8	1	1	100	1
Strain	ALK2	M1442	M1442	ALK2	M2886	M2886	M2886
Initial concentrations
Solids (%)	16.5	16.5	16.5	12.0	12.0	12.0	0.0
Cellulose (g/L)	100	100	100	64.5	64.5	64.5	0.0
Glucose (g/L)	20	20	20	1.0	1.0	1.2	88.6
Xylose (g/L)	35	35	35	13.9	13.9	16.6	24.3
Other sugars (g/L)				3.8	3.8	3.2	5.2
Acetic acid and other inhibitors (g/L)	10.5	10.5	10.0	6.3	6.3	6.3	4.5
Fermentation performance
Fermentation time (hours)	97	93	90	60	60	60	60
Final ethanol titer (g/L)	45.7	61.4	54.7	32.6	32.0	30.8	49.5
Cellulose conversion (%)	71.0	75.1	83.4	83.6	80.4	77.2	n/a
Glucose utilization (%)	79.5	93.6	85.9	97.2	94.7	99.5	89.3
Xylose utilization (%)	58.1	99.6	80.9	82.3	86.7	100.0	91.6
Metabolic yield (%)	90.5	91.5	85.8	75.9	81.0	78.4	90.1