Binding mode of an overlay of 1 with the most potent analogs (5, 8, 10, 11, 13, 14, 38, and 58—with omission of 24) to the spermine binding site of GluN1 in the Low Po mode. Six amino acid residues of GluN1 known to influence spermine stimulation and the bisbenzamidines are represented as sticks with carbon atoms in coral and green, respectively. Surface representation of 1 is colored light blue.